Jpet # 162222 1 Persistent Induction of Cytochrome P4501a1 in Human Hepatoma Cells by 3-methylcholanthrene: Evidence for Sustained Transcriptional Activation of the Cyp1a1 Promoter

نویسندگان

  • INAYAT S. FAZILI
  • WEIWU JIANG
  • LIHUA WANG
  • EDWARD A. FELIX
چکیده

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Persistent induction of cytochrome P4501A1 in human hepatoma cells by 3-methylcholanthrene: evidence for sustained transcriptional activation of the CYP1A1 promoter.

Cytochrome P450 (P450)1A1 plays a critical role in the metabolic activation and detoxification of polycyclic aromatic hydrocarbons (PAHs), many of which are potent human carcinogens. In this investigation, we tested the hypothesis that MC elicits persistent induction of CYP1A1 expression in human hepatoma cells (HepG2) and that this phenomenon is mediated by sustained transcriptional activation...

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Differential regulation of expression of hepatic and pulmonary cytochrome P4501A enzymes by 3-methylcholanthrene in mice lacking the CYP1A2 gene.

The cytochrome P4501A enzymes play important roles in carcinogen metabolism. We reported previously that 3-methylcholanthrene (MC) elicits a persistent induction of hepatic, pulmonary, and mammary microsomal cytochrome P450 (P450) 1A enzymes for several weeks after MC withdrawal. In this study, we tested the hypothesis that CYP1A2, a liver-specific P450 isozyme, plays an important role in the m...

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Induction of eytochrome P4501A1: a model for analyzing mammalian gene transcription

The induction of microsomal cytochrome P4501A1 by polycycic aromatic hydrocarbons represents an interesting response by which mammalian cells adapt to xenobiotic exposure. Enzyme induction reflects increased transcription of the corresponding CYP1A1 gene. Analyses of the induction mechanism using genetic, biochemical, and molecular biological approaches have revealed a novel transcriptional reg...

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تاریخ انتشار 2010